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TheiaProk Inputs on Terra

When running tbp-parser as part of the TheiaProk workflow series (find documentation for TheiaProk here) on Terra.bio, an optional input must be activated to instruct TheiaProk to run tbp-parser.

tbp-parser is not on by default due to the nature of this tool and its outputs.

TheiaProk Version

This information only corresponds to PHB v2.2.0. These inputs and outputs may not be applicable to other versions of TheiaProk.

Required Inputs

To activate tbp-parser you must set the following variable to true:

Terra Task name Variable Type Default value Description
merlin_magic tbprofiler_additional_outputs Boolean false Set to true to activate tbp-parser

Optional Inputs

The following optional inputs are also available for user modification on Terra:

Terra Task name Variable Type Default value Description
merlin_magic tbp_parser_output_seq_method_type String "WGS" Fills out the “seq_method” field in the tbp_parser output files
merlin_magic tbp_parser_operator String "Operator not provided" The operator who ran the analysis; used in the LIMS & Looker reports
merlin_magic tbp_parser_min_depth Int 10 The minimum depth of coverage required for a site to pass QC
merlin_magic tbp_parser_min_frequency Int 0.1 The minimum frequency for a mutation to pass QC (0.1 -> 10%)
merlin_magic tbp_parser_min_read_support Int 10 The minimum read support for a mutation to pass QC
merlin_magic tbp_parser_coverage_threshold Int 100 The minimum percentage of a region that has depth above the threshold set by min_depth (used for a gene/locus to pass QC)
merlin_magic tbp_parser_coverage_regions_bed File tbdb-modified-regions.md A BED file containing the regions to calculate percent coverage for
merlin_magic tbp_parser_debug Boolean false Turn on debug mode for tbp-parser
merlin_magic tbp_parser_add_cs_lims Boolean false Adds Cycloserine (CS) fields to the LIMS report
merlin_magic tbp_parser_docker_image String "us-docker.pkg.dev/general-theiagen/theiagen/tbp-parser:1.6.0" The Docker image to use when running tbp-parser

Find the outputs for tbp-parser in TheiaProk on Terra here.